Automated KAPA Library preparation Kit on ACSIA

Kit KAPA Roche
3 December 2015

The libraries preparation steps are long and repetitive. The automation of this preparation permits to avoid any human error and improve the quality of the libraries thanks to greater repeatability and more accurate pipetting.
PrimaDiag developed the NGS system based on ACSIA platform using the latest technologies which allow the handling of magnetic beadstemperature control with 0.1°C of accuracy and shaking from 200 to 3000 rpm. This system fits with any kind of plate and deepwell available on the market (no skirted, semi skirted, skirted plates and so on). Moreover, all liquid handling parameters are carefully optimized for minimal reagents consumption and the protocols are optimized to use a minimum number of tips (3 tip boxes for preparing 48 libraries instead of 14).

Find In this Application note, the methods used on ACSIA NGS Edition and a comparative evaluation between results obtained manually and on the PrimaDiag platform with the KAPA Library Preparation Kit from Kapa Biosystems.

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