Validation of Nextera DNA Sample Library Preparation Kit

Groupement Hospitalier de la Pitié Salpêtrière
15 October 2015

Since its release in August 2014, ACSIA NGS Edition continues to evolve and expand its portfolio of automated kits (NEB Next Ultra, KAPA Library Preparation, Agilent SureSelect, NimbleGen SeqCap EZ library …).

With the help of  The Molecular and Chromosomic Genetics of the Pitié Salpêtrière Hospital group, we optimized the critical parameters used  in the step of tagmentation (Basic principle of Illumina Nextera DNA Sample Library Preparation Kit).

With optimized parameters, our team  validated the Illumina Kit on ACSIA NGS Edition  with excellent performance. For example, at the end of the process, the yield with ACSIA NGS Edition is twice that with manual treatment.

Manual : 

Traitement ACSIA NGS Edition   

  • Main peak at 334 Bp
  • Pre-PCR Concentration (CLARIOstar) → 384 pg/µL

ACSIA NGS Edition :

Traitement manuel

  • Main peak at 297 Bp
  • Pre-PCR Concentration (CLARIOstar) → 852 pg/µL

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