Applications de l’ACSIA: Nos Protocoles Automatisés
Les protocoles suivants ont été automatisés par PRIMADIAG pour l’ACSIA :
| Manufacturer | Kit | Sample max/run |
| Illumina | TruSight HLA | 12 |
| Nextera | 96 | |
| TruSeq Stranded Total RNA | 48 | |
| TruSeq Stranded mRNA | 48 | |
| Nextera XT Pre et Post | 48 | |
| TruSight Rapid Capture | 48 | |
| Metagenomic | 96 | |
| TruSeq nano DNA | 24 | |
| Thermo-Fisher | AmpliSeq | 48 |
| NEB | NEB NextUltra DNA | 96 |
| Roche | KAPA DNA | 48 |
| KAPA Hyper Plus | 48 | |
| NimbleGen – Capture (SeqCapEZ) | 48 | |
| Agilent | Agilent SureSelect XT – Pre | 32 |
| Agilent SureSelect QXT – Pre | 48 | |
| Agilent SureSelect XT (or QXT) – Capture | 48 |
Pre-PCR
TruSight HLA
| ACSIA | Manual | |
| Number sample max | 12 (8 reactions by sample) | |
| Number of tips used by sample | 63 | 152 |
| Hand-on time | 15 minutes | 3h |
| Duration of workflow | 3h | 3h |
Nextera
| ACSIA | Manual | |
| Number sample max | 12 (8 reactions by sample) | |
| Number of tips used by sample | 63 | 152 |
| Hand-on time | 15 minutes | 3h |
| Duration of workflow | 3h | 3h |
Nextera XT Pre
| ACSIA | Manual | |
| Number sample max | 48 | |
| Number of tips used by sample | 2 | 2 |
| Hand-on time | 10 minutes | 45 minutes |
| Duration of workflow | 45 minutes | 45 minutes |
Metagenomic Pre
| ACSIA | Manual | |
| Number sample max | 96 | |
| Number of tips used by sample | 0.3 | 1 |
| Hand-on time | 5 minutes | 20 minutes |
| Duration of workflow | 20 minutes | 20 minutes |
NEB NextUltra DNA
| ACSIA | Manual | |
| Number sample max | 96 | |
| Number of tips used by sample | 5 | 12 |
| Hand-on time | 10 minutes | 3 hours |
| Duration of workflow | 3h30 | 3h30 |
Kapa DNA
| ACSIA | Manual | |
| Number sample max | 48 | |
| Number of tips used by sample | 6 | 28 |
| Hand-on time | 15 minutes | 4h |
| Duration of workflow | 5h | 5h |
KAPA Hyper Plus
| ACSIA | Manual | |
| Number sample max | 48 | |
| Number of tips used by sample | 9 | 24 |
| Hand-on time | 30minutes | 4h15 |
| Duration of workflow | 4h15 | 4h15 |
TruSeq Stranded Total RNA
| ACSIA | Manual | |
| Number sample max | 48 | |
| Number of tips used by sample | 8 | 31 |
| Hand-on time | 20 minutes | 4h |
| Duration of workflow | 7 h | 7 h |
TruSeq Stranded mRNA
| ACSIA | Manual | |
| Number sample max | 48 | |
| Number of tips used by sample | 12 | 39 |
| Hand-on time | 15 | 6h |
| Duration of workflow | 6h | 6h |
TruSeq Nano DNA
| ACSIA | Manual | |
| Number sample max | 24 | |
| Number of tips used by sample | 16 | 32 |
| Hand-on time | 30 minutes | 6h |
| Duration of workflow | 6 h | 6 h |
Agilent SureSelect XT Fast
| ACSIA | Manual | |
| Number sample max | 32 | |
| Number of tips used by sample | 9 | 26 |
| Hand-on time | 15 | 2h30 |
| Duration of workflow | 3h50 | 3h50 |
Agilent SureSelect QXT
| ACSIA | Manual | |
| Number sample max | 96 | |
| Number of tips used by sample | 5 | 12 |
| Hand-on time | 15 | 2h30 |
| Duration of workflow | 3h20 | 3h20 |
Post-PCR
NimbleGen Capture
| ACSIA | Manual | |
| Number multiplex max | 48 | |
| Number of tips used by sample | 4,5 | 14 |
| Hand-on time | 15 minutes | 3h15 |
| Duration of workflow | 4h | 4h |
TruSight Rapid Capture
| ACSIA | Manual | |
| Number sample max | 48 | |
| Number of tips used by sample | 5×2 | 7×2 |
| Hand-on time | 15minutes | 3h |
| Duration of workflow | 3h | 3h |
SureSelect XT(QXT) Capture
| ACSIA | Manual | |
| Number sample max | 96 | |
| Number of tips used by sample | 4 | 8 |
| Hand-on time | 15 minutes | 3 h30 |
| Duration of workflow | 3h30 | 3h30 |
Nextera XT Post
| ACSIA | Manual | |
| Number sample max | 48 | |
| Number of tips used by sample | 7.5 | 16 |
| Hand-on time | 20minutes | 3h |
| Duration of workflow | 3h | 3h |
AmpliSeq
| ACSIA | Manual | |
| Number sample max | 48 | |
| Number of tips used by sample | 8 | 15 |
| Hand-on time | 15minutes | 3h40 |
| Duration of workflow | 3h40 | 3h40 |
