Applications de l’ACSIA: Nos Protocoles Automatisés

15 mai 2018

Les protocoles suivants ont été automatisés par PRIMADIAG pour l’ACSIA :

Manufacturer Kit Sample max/run
Illumina TruSight HLA 12
Nextera 96
TruSeq Stranded Total RNA 48
TruSeq Stranded mRNA 48
Nextera XT Pre et Post 48
TruSight Rapid Capture 48
Metagenomic 96
TruSeq nano DNA 24
Thermo-Fisher AmpliSeq 48
NEB NEB NextUltra DNA 96
Roche KAPA DNA 48
KAPA Hyper Plus 48
NimbleGen – Capture (SeqCapEZ) 48
Agilent Agilent SureSelect XT – Pre 32
Agilent SureSelect QXT – Pre 48
Agilent SureSelect XT (or QXT) – Capture 48

Pre-PCR

TruSight HLA

ACSIA Manual
Number sample max 12 (8 reactions by sample)
Number of tips used by sample 63 152
Hand-on time 15 minutes 3h
Duration of workflow 3h 3h

Nextera

ACSIA Manual
Number sample max 12 (8 reactions by sample)
Number of tips used by sample 63 152
Hand-on time 15 minutes 3h
Duration of workflow 3h 3h

Nextera XT Pre

ACSIA Manual
Number sample max 48
Number of tips used by sample 2 2
Hand-on time 10 minutes 45 minutes
Duration of workflow 45 minutes 45 minutes

Metagenomic Pre

ACSIA Manual
Number sample max 96
Number of tips used by sample 0.3 1
Hand-on time 5 minutes 20 minutes
Duration of workflow 20 minutes 20 minutes

NEB NextUltra DNA

ACSIA Manual
Number sample max 96
Number of tips used by sample 5 12
Hand-on time 10 minutes 3 hours
Duration of workflow 3h30 3h30

Kapa DNA

ACSIA Manual
Number sample max 48
Number of tips used by sample 6 28
Hand-on time 15 minutes 4h
Duration of workflow 5h 5h

KAPA Hyper Plus

ACSIA Manual
Number sample max 48
Number of tips used by sample 9 24
Hand-on time 30minutes 4h15
Duration of workflow 4h15 4h15

TruSeq Stranded Total RNA

ACSIA Manual
Number sample max 48
Number of tips used by sample 8 31
Hand-on time 20 minutes 4h
Duration of workflow 7 h 7 h

TruSeq Stranded mRNA

ACSIA Manual
Number sample max 48
Number of tips used by sample 12 39
Hand-on time 15 6h
Duration of workflow 6h 6h

TruSeq Nano DNA

ACSIA Manual
Number sample max 24
Number of tips used by sample 16 32
Hand-on time 30 minutes 6h
Duration of workflow 6 h 6 h

Agilent SureSelect XT Fast

ACSIA Manual
Number sample max 32
Number of tips used by sample 9 26
Hand-on time 15 2h30
Duration of workflow 3h50 3h50

Agilent SureSelect QXT

ACSIA Manual
Number sample max 96
Number of tips used by sample 5 12
Hand-on time 15 2h30
Duration of workflow 3h20 3h20

Post-PCR

NimbleGen Capture

ACSIA Manual
Number multiplex max 48
Number of tips used by sample 4,5 14
Hand-on time 15 minutes 3h15
Duration of workflow 4h 4h

TruSight Rapid Capture

ACSIA Manual
Number sample max 48
Number of tips used by sample 5×2 7×2
Hand-on time 15minutes 3h
Duration of workflow 3h 3h

SureSelect XT(QXT) Capture

ACSIA Manual
Number sample max 96
Number of tips used by sample 4 8
Hand-on time 15 minutes 3 h30
Duration of workflow 3h30 3h30

Nextera XT Post

ACSIA Manual
Number sample max 48
Number of tips used by sample 7.5 16
Hand-on time 20minutes 3h
Duration of workflow 3h 3h

AmpliSeq

ACSIA Manual
Number sample max 48
Number of tips used by sample 8 15
Hand-on time 15minutes 3h40
Duration of workflow 3h40 3h40

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